Which culture medium is used to selectively grow the fused hybridoma cells?

Options :
  1. HAT medium
  2. Agar-Agar medium
  3. RPMI 20%
  4. None of the above

Answer and Explanation :-

Answer: Option 1


The selective culture medium for hybridoma cells is called HAT medium because it contains hypoxanthine, aminopterin, and thymidine. This medium is selective for fused hybridoma cells. Unfused myeloma cells cannot grow because they lack HGPRT, and thus cannot replicate their DNA.

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